Part:BBa_K5048055:Design

plasmid pBAD luxR lasl

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2082
  Illegal EcoRI site found at 2874
  Illegal EcoRI site found at 3443
  Illegal PstI site found at 2270
  Illegal PstI site found at 3062
  Illegal PstI site found at 3423
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2082
  Illegal EcoRI site found at 2874
  Illegal EcoRI site found at 3443
  Illegal NheI site found at 897
  Illegal NheI site found at 920
  Illegal NheI site found at 1839
  Illegal NheI site found at 1862
  Illegal NheI site found at 2631
  Illegal NheI site found at 2654
  Illegal PstI site found at 2270
  Illegal PstI site found at 3062
  Illegal PstI site found at 3423
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2082
  Illegal EcoRI site found at 2874
  Illegal EcoRI site found at 3443
  Illegal BglII site found at 3419
  Illegal BamHI site found at 3409
  Illegal XhoI site found at 3415
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2082
  Illegal EcoRI site found at 2874
  Illegal EcoRI site found at 3443
  Illegal PstI site found at 2270
  Illegal PstI site found at 3062
  Illegal PstI site found at 3423
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2082
  Illegal EcoRI site found at 2874
  Illegal EcoRI site found at 3443
  Illegal PstI site found at 2270
  Illegal PstI site found at 3062
  Illegal PstI site found at 3423
  Illegal NgoMIV site found at 2336
  Illegal NgoMIV site found at 3128
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 216
  Illegal BsaI site found at 3622
  Illegal BsaI site found at 4689
  Illegal SapI.rc site found at 5771
  

Design Notes

We replace the T7 promoter with araBAD promoter to prevent the leakage of promoter

Source

complete synthesis